MTHFR (P42898) variants and mutations
MTHFR (also known as P42898) is a human protein-coding gene encoding a methylenetetrahydrofolate reductase (NADPH) protein. It generates 5-methyltetrahydrofolate for remethylation of homocysteine to methionine, linking folate metabolism with methyl-group supply. Severe biallelic deficiency causes homocystinuria with neurologic and thrombotic complications, while common variants produce much smaller effects on homocysteine. This analysis covers 1,345 MTHFR variants and mutations. Of these, 58% have computational variant effect predictions. Disease context includes homocystinuria due to methylene tetrahydrofolate reductase deficiency, schizophrenia, and neural tube defects, folate-sensitive. Example MTHFR variants include M1?, M1K, and V2A.
Variant analysis overview
- Gene: MTHFR
- Protein: P42898
- UniProt accession: P42898
- Organism: Homo sapiens
- Variants analyzed: 1345
- Variant scope: all variants
- Completed: 2026-08-21
Variant and mutation evidence
- Variant composition: 1,164 unspecified-consequence records; 2 stop lost; 53 synonymous variants; 103 missense variants; 12 frameshift variants; 7 in-frame deletions; 5 stop-gained variants; 1 in-frame insertions; 1 splice-region variants; 2 substitution
- Prediction scores: 785 variants have prediction scores (58% of the analyzed set).
Clinical, disease, and population context
- Disease context: 25 disease associations are represented. Top associations: homocystinuria due to methylene tetrahydrofolate reductase deficiency, schizophrenia, neural tube defects, folate-sensitive, thrombophilia due to thrombin defect, Global developmental delay, Bilateral tonic-clonic seizure, ischemic stroke, isolated spina bifida, Seizure, Abnormality of metabolism/homeostasis, Mental deterioration, infantile spasms.
Protein structure and variant hotspots
- Protein features: 19 binding sites; 16 post-translational modification sites.
- PTM context: 21 variants overlap post-translational modification sites.
Data sources
Evidence in this analysis draws on EBI Proteins Variation, UniProt, gnomAD v4, EuropePMC, 3D Hotspot Analysis, Interaction Network Analysis, Protein Data Bank, AlphaFold DB, gnomAD constraint, Open Targets, ClinGen, MaveDB, LitVar.
Notable MTHFR variants
Examples include M1?, M1K, V2A, V2M, N3K, E4K, E4Q, R6G. Listed records include available protein-change notation, database identifiers, clinical classifications, computational predictions, population evidence, experimental measurements, and disease context.
- M1?, rs373076763, ClinGen CA595764, cosmic curated COSV56742, ClinVar RCV000594799, Likely pathogenic
- M1K (p.Met1Lys), rs1644429362, ClinGen CA338423386, ClinVar RCV003093571, Pathogenic, Homocystinuria due to methylene tetrahydrofolate reductase deficiency
- V2A (p.Val2Ala), TOPMed rs1332859061, gnomAD rs1332859061, REVEL 0.49, CADD 18.90
- V2M (p.Val2Met), rs760971509, ClinGen CA595763, ClinVar RCV003522430, ExAC rs760971509, REVEL 0.37, CADD 22.10, Uncertain significance, Homocystinuria due to methylene tetrahydrofolate reductase deficiency
- N3K (p.Asn3Lys), cosmic curated COSV56742, REVEL 0.28, CADD 6.50
- E4K (p.Glu4Lys), rs772252042, ClinGen CA595761, ClinVar RCV003095698, ExAC rs772252042, REVEL 0.38, CADD 22.60, Uncertain significance, Homocystinuria due to methylene tetrahydrofolate reductase deficiency
- E4Q (p.Glu4Gln), ExAC rs772252042, TOPMed rs772252042, gnomAD rs772252042, REVEL 0.32, CADD 21.00, Uncertain significance, Inborn genetic diseases
- R6G (p.Arg6Gly), NCI-TCGA Cosmic COSV5673, cosmic curated COSV56739, Variant assessed as somatic; moderate impact.
- R6K (p.Arg6Lys), rs2522982484, ClinGen CA338423353, ClinVar RCV002816226, REVEL 0.15, CADD 12.50, Uncertain significance, Homocystinuria due to methylene tetrahydrofolate reductase deficiency
- R6S (p.Arg6Ser), ExAC rs748686763, TOPMed rs748686763, gnomAD rs748686763, REVEL 0.21, CADD 12.80, Likely benign
- R6T (p.Arg6Thr), cosmic curated COSV56738
- G7E (p.Gly7Glu), cosmic curated COSV10609, TOPMed rs925247064, gnomAD rs925247064, REVEL 0.27, CADD 19.70
- N8S (p.Asn8Ser), NCI-TCGA TCGA novel, Variant assessed as somatic; moderate impact.
- S10N (p.Ser10Asn), 1000Genomes rs143428827, ESP rs143428827, ExAC rs143428827, TOPMed rs143428827, REVEL 0.25, CADD 14.00, Uncertain significance
- S10T (p.Ser10Thr), rs143428827, ClinGen CA595758, ClinVar RCV002610149, ClinVar RCV004654143, REVEL 0.24, CADD 13.60, Conflicting interpretations, not provided; Inborn genetic diseases; Homocystinuria due to methylene tetrahydr
- L11F (p.Leu11Phe), ExAC rs768905024, gnomAD rs768905024, REVEL 0.25, CADD 5.45
- N12D (p.Asn12Asp), gnomAD rs1644428324, REVEL 0.25, CADD 0.16
- N12K (p.Asn12Lys), rs1046343781, ClinGen CA17975344, ClinVar RCV003104214, ClinVar RCV003313312, REVEL 0.19, CADD 1.79, Uncertain significance, not provided; Homocystinuria due to methylene tetrahydrofolate reductase deficie
- P13H (p.Pro13His), cosmic curated COSV56738
- P13L (p.Pro13Leu), cosmic curated COSV10041, ExAC rs749606539, gnomAD rs749606539, REVEL 0.25, CADD 17.30
- C14F (p.Cys14Phe), Ensembl rs1644428085, REVEL 0.18, CADD 1.85
- L15S (p.Leu15Ser), TOPMed rs1417143049, gnomAD rs1417143049, REVEL 0.24, CADD 3.90
- E16G (p.Glu16Gly), rs780279740, ClinGen CA595755, ClinVar RCV004506677, ExAC rs780279740, REVEL 0.26, CADD 16.50, Uncertain significance, Inborn genetic diseases
- E16Q (p.Glu16Gln), TOPMed rs1557771398, gnomAD rs1557771398, REVEL 0.26, CADD 11.20
- G17D (p.Gly17Asp), Ensembl rs1570498463, REVEL 0.56, CADD 20.60, Uncertain significance, Homocystinuria due to methylene tetrahydrofolate reductase deficiency
- A19V (p.Ala19Val), gnomAD rs1333552513, REVEL 0.24, CADD 15.60
- S20G (p.Ser20Gly), rs1410562479, ClinGen CA338423261, ClinVar RCV002605427, gnomAD rs1410562479, Uncertain significance, Homocystinuria due to methylene tetrahydrofolate reductase deficiency
- S20N (p.Ser20Asn), cosmic curated COSV10516
- S21I (p.Ser21Ile), 1000Genomes rs372125653, ExAC rs372125653, TOPMed rs372125653, gnomAD rs372125653, Uncertain significance
- S21N (p.Ser21Asn), rs372125653, ClinGen CA595753, ClinVar RCV002633333, 1000Genomes rs372125653, REVEL 0.41, CADD 21.40, Uncertain significance, Homocystinuria due to methylene tetrahydrofolate reductase deficiency
- S21R (p.Ser21Arg), TOPMed rs1215184376, gnomAD rs1215184376, REVEL 0.42, CADD 20.60
- S23I (p.Ser23Ile), ExAC rs777286995, TOPMed rs777286995, gnomAD rs777286995, REVEL 0.30, CADD 20.00
- S23N (p.Ser23Asn), ExAC rs777286995, TOPMed rs777286995, gnomAD rs777286995, REVEL 0.26, CADD 17.10
- S23R (p.Ser23Arg), cosmic curated COSV56738
- E24D (p.Glu24Asp), ExAC rs752585350, gnomAD rs752585350, REVEL 0.37, CADD 23.50
- E24G (p.Glu24Gly), Ensembl rs184902174
- S26T (p.Ser26Thr), cosmic curated COSV10041
- K27E (p.Lys27Glu), TOPMed rs1484182630, gnomAD rs1484182630, REVEL 0.29, CADD 22.90
- D28N (p.Asp28Asn), rs2100578318, ClinGen CA338423206, ClinVar RCV001758102, Ensembl rs2100578318, REVEL 0.27, CADD 22.50, Uncertain significance, not provided
- S29G (p.Ser29Gly), TOPMed rs1235705216, gnomAD rs1235705216, REVEL 0.28, CADD 22.70
- S30L (p.Ser30Leu), rs886043815, ClinGen CA10605985, NCI-TCGA Cosmic COSV1004, cosmic curated COSV10041, REVEL 0.48, CADD 32.00, Uncertain significance, not provided
- R31S (p.Arg31Ser), gnomAD rs762296846, REVEL 0.54, CADD 22.80
- C32Y (p.Cys32Tyr), cosmic curated COSV10516
- S33Y (p.Ser33Tyr), cosmic curated COSV10461
- T34I (p.Thr34Ile), rs753730796, ClinGen CA595747, ClinVar RCV003058563, ExAC rs753730796, REVEL 0.43, CADD 24.10, Uncertain significance, Homocystinuria due to methylene tetrahydrofolate reductase deficiency
- T34P (p.Thr34Pro), ExAC rs759381630, gnomAD rs759381630
- T34S (p.Thr34Ser), ExAC rs759381630, gnomAD rs759381630, REVEL 0.29, CADD 20.40
- P35L (p.Pro35Leu), cosmic curated COSV56738, ExAC rs766242179, gnomAD rs766242179, REVEL 0.49, CADD 30.00
- P35Q (p.Pro35Gln), cosmic curated COSV10041
- G36A (p.Gly36Ala), ESP rs376266183, ExAC rs376266183, TOPMed rs376266183, gnomAD rs376266183, Uncertain significance
- G36D (p.Gly36Asp), cosmic curated COSV56742
- G36V (p.Gly36Val), rs376266183, ClinGen CA595743, ClinVar RCV002604176, ClinVar RCV005674997, REVEL 0.16, CADD 18.30, Uncertain significance, Inborn genetic diseases; Homocystinuria due to methylene tetrahydrofolate reduct
- D38H (p.Asp38His), ExAC rs761916311, TOPMed rs761916311, gnomAD rs761916311, REVEL 0.49, CADD 25.00, Uncertain significance, Neural tube defects, folate-sensitive; Thrombophilia due to thrombin defect; Hom
- D38N (p.Asp38Asn), ExAC rs761916311, TOPMed rs761916311, gnomAD rs761916311
- P39H (p.Pro39His), cosmic curated COSV10041
- E40K (p.Glu40Lys), NCI-TCGA Cosmic COSV5674, cosmic curated COSV56742, REVEL 0.37, CADD 21.80, Variant assessed as somatic; moderate impact.
- E40Q (p.Glu40Gln), TOPMed rs1644425771, gnomAD rs1644425771, REVEL 0.38, CADD 21.60
- R41Q (p.Arg41Gln), cosmic curated COSV56741, ExAC rs775972969, TOPMed rs775972969, gnomAD rs775972969, REVEL 0.34, CADD 24.00
- R41W (p.Arg41Trp), rs749729349, NCI-TCGA Cosmic COSV1004, cosmic curated COSV10041, ExAC rs749729349, REVEL 0.53, CADD 28.70, Variant assessed as somatic; moderate impact.
- E43D (p.Glu43Asp), NCI-TCGA Cosmic COSV5674, cosmic curated COSV56742, Variant assessed as somatic; moderate impact.
- R44K (p.Arg44Lys), gnomAD rs1395556961
- L45F (p.Leu45Phe), NCI-TCGA TCGA novel, Variant assessed as somatic; moderate impact.
- R46P (p.Arg46Pro), ExAC rs776483190, TOPMed rs776483190, gnomAD rs776483190, REVEL 0.51, CADD 20.70, Likely pathogenic, in MTHFRD
- R46Q (p.Arg46Gln), rs776483190, ClinGen CA198559, ClinVar RCV000167587, ClinVar RCV003468812, REVEL 0.27, CADD 18.30, Likely pathogenic, Neural tube defects, folate-sensitive; Homocystinuria due to methylene tetrahydr
- R46W (p.Arg46Trp), rs138189536, ClinGen CA198556, cosmic curated COSV10942, ClinVar RCV000167586, REVEL 0.52, CADD 24.70, Conflicting interpretations, not specified; Homocystinuria due to methylene tetrahydrofolate reductase defici
- K48R (p.Lys48Arg), TOPMed rs992117198, gnomAD rs992117198, REVEL 0.71, CADD 25.10
- M49V (p.Met49Val), rs1570498105, ClinGen CA338423075, ClinVar RCV000822910, Ensembl rs1570498105, Uncertain significance, Homocystinuria due to methylene tetrahydrofolate reductase deficiency
- R51G (p.Arg51Gly), rs764131110, ClinGen CA338423060, ClinVar RCV002892532, REVEL 0.79, CADD 29.30, Uncertain significance, Inborn genetic diseases
- R51L (p.Arg51Leu), cosmic curated COSV56738, 1000Genomes rs201618781, ESP rs201618781, ExAC rs201618781, REVEL 0.67, CADD 24.20
- R51P (p.Arg51Pro), rs201618781, UniProt VAR 009530, 1000Genomes rs201618781, ESP rs201618781, REVEL 0.83, CADD 26.30, Likely pathogenic, Homocystinuria due to methylene tetrahydrofolate reductase deficiency
- R51Q (p.Arg51Gln), cosmic curated COSV56742, 1000Genomes rs201618781, ESP rs201618781, ExAC rs201618781, REVEL 0.55, CADD 24.20
- R51W (p.Arg51Trp), rs764131110, ClinGen CA241600, ClinVar RCV000175816, ClinVar RCV002478570, REVEL 0.77, CADD 32.00, Uncertain significance, Schizophrenia; Thrombophilia due to thrombin defect; Homocystinuria due to methy
- R52* (p.Arg52Ter), rs986604359, ClinGen CA17975257, NCI-TCGA Cosmic COSV5673, cosmic curated COSV56739, CADD 36.00, Pathogenic, in MTHFRD
- R52P (p.Arg52Pro), ExAC rs754980119, TOPMed rs754980119, gnomAD rs754980119, REVEL 0.87, CADD 27.20, Pathogenic, in MTHFRD
- R52Q (p.Arg52Gln), rs754980119, ClinGen CA595734, cosmic curated COSV56738, ClinVar RCV000690846, REVEL 0.76, CADD 23.30, Pathogenic/Likely pathogenic, not provided; Neural tube defects, folate-sensitive; Homocystinuria due to methy
- E54D (p.Glu54Asp), rs753692902, ClinGen CA338423043, ClinVar RCV001947633, ExAC rs753692902, REVEL 0.26, CADD 3.01, Uncertain significance, Homocystinuria due to methylene tetrahydrofolate reductase deficiency
- E54K (p.Glu54Lys), cosmic curated COSV56739, TOPMed rs1196257926, gnomAD rs1196257926, REVEL 0.43, CADD 23.10
- E54Q (p.Glu54Gln), cosmic curated COSV56742
- G56A (p.Gly56Ala), ExAC rs766295185, gnomAD rs766295185, REVEL 0.81, CADD 26.30
- D57N (p.Asp57Asn), ExAC rs755932641, TOPMed rs755932641, gnomAD rs755932641, REVEL 0.46, CADD 26.50
- K58Q (p.Lys58Gln), ExAC rs750677509, gnomAD rs750677509, REVEL 0.28, CADD 22.70
- W59* (p.Trp59Ter), rs767789270, ClinGen CA338423012, ClinVar RCV001939988, ExAC rs767789270, CADD 39.00, Pathogenic, in MTHFRD
- W59C (p.Trp59Cys), rs767789270, ExAC rs767789270, gnomAD rs767789270, ClinGen CA595729, REVEL 0.85, CADD 32.00, Conflicting interpretations, Homocystinuria due to methylene tetrahydrofolate reductase deficiency
- W59S (p.Trp59Ser), rs786204007, ClinGen CA198562, ClinVar RCV000167588, UniProt VAR 074113, Pathogenic, Homocystinuria due to methylene tetrahydrofolate reductase deficiency
- F60L (p.Phe60Leu), Ensembl rs1557771026
- S61F (p.Ser61Phe), cosmic curated COSV56738
- L62M (p.Leu62Met), cosmic curated COSV10041
- E63K (p.Glu63Lys), ExAC rs774531580, gnomAD rs774531580
- F65L (p.Phe65Leu), TOPMed rs1305385953, gnomAD rs1305385953, REVEL 0.90, CADD 32.00
- P66L (p.Pro66Leu), rs796064512, ClinGen CA204396, ClinVar RCV000190392, Ensembl rs796064512, not provided, Homocystinuria due to methylene tetrahydrofolate reductase deficiency
- P66S (p.Pro66Ser), cosmic curated COSV10461
- P66T (p.Pro66Thr), cosmic curated COSV10041
- P67S (p.Pro67Ser), rs1644422806, ClinGen CA338422961, ClinVar RCV001264802, Ensembl rs1644422806, REVEL 0.97, CADD 28.80, Uncertain significance, Homocystinuria due to methylene tetrahydrofolate reductase deficiency
- R68* (p.Arg68Ter), rs763539350, ClinGen CA595726, ClinVar RCV001380126, ClinVar RCV002305606, CADD 38.00, Pathogenic
- R68G (p.Arg68Gly), rs763539350, ClinGen CA198565, ClinVar RCV000167589, ClinVar RCV003462246, REVEL 0.88, CADD 27.40, Pathogenic/Likely pathogenic, Schizophrenia; Thrombophilia due to thrombin defect; Homocystinuria due to methy
- R68Q (p.Arg68Gln), rs2066472, ClinGen CA595725, cosmic curated COSV10041, ClinVar RCV001958245, REVEL 0.69, CADD 31.00, Uncertain significance, Thrombophilia due to thrombin defect; Homocystinuria due to methylene tetrahydro
- T69A (p.Thr69Ala), ExAC rs770151367, gnomAD rs770151367, REVEL 0.87, CADD 27.90
- T69I (p.Thr69Ile), ExAC rs746177570, TOPMed rs746177570, gnomAD rs746177570, REVEL 0.92, CADD 28.80, Uncertain significance, Inborn genetic diseases
- T69N (p.Thr69Asn), cosmic curated COSV56738
- G72* (p.Gly72Ter), NCI-TCGA Cosmic COSV1004, cosmic curated COSV10041, Variant assessed as somatic; high impact.
- G72E (p.Gly72Glu), cosmic curated COSV56742, gnomAD rs1644422409, REVEL 0.75, CADD 25.70
- V74L (p.Val74Leu), cosmic curated COSV56739
- L76F (p.Leu76Phe), Ensembl rs1644422349
- I77V (p.Ile77Val), gnomAD rs1347839321, REVEL 0.26, CADD 21.80
- S78* (p.Ser78Ter), rs776969786, ClinGen CA17975236, ClinVar RCV000986238, ClinVar RCV001236475, Pathogenic
- S78L (p.Ser78Leu), ExAC rs776969786, TOPMed rs776969786, gnomAD rs776969786, REVEL 0.61, CADD 24.70, Pathogenic
- R79=, NCI-TCGA TCGA novel, Variant assessed as somatic; low impact.
- R79K (p.Arg79Lys), cosmic curated COSV10735
- R79S (p.Arg79Ser), rs1553187509, ClinGen CA338422769, ClinVar RCV000625779, Ensembl rs1553187509, REVEL 0.73, CADD 29.10, Likely pathogenic, Homocystinuria due to methylene tetrahydrofolate reductase deficiency
- F80L (p.Phe80Leu), rs541505625, ClinGen CA17974728, ClinVar RCV001243849, 1000Genomes rs541505625, REVEL 0.55, CADD 22.80, Uncertain significance, Homocystinuria due to methylene tetrahydrofolate reductase deficiency
- D81G (p.Asp81Gly), gnomAD rs1261482213, REVEL 0.88, CADD 26.00
- R82Q (p.Arg82Gln), rs778417385, ClinGen CA595704, NCI-TCGA Cosmic COSV5673, cosmic curated COSV56738, REVEL 0.34, CADD 21.10, Uncertain significance, Homocystinuria due to methylene tetrahydrofolate reductase deficiency
- R82W (p.Arg82Trp), rs786204009, ClinGen CA198568, ClinVar RCV000167590, ClinVar RCV005429226, REVEL 0.84, CADD 32.00, Uncertain significance, not provided; not specified
- M83T (p.Met83Thr), rs1426036757, ClinGen CA338422729, ClinVar RCV000692160, TOPMed rs1426036757, REVEL 0.95, CADD 24.10, Uncertain significance, Homocystinuria due to methylene tetrahydrofolate reductase deficiency
- A85G (p.Ala85Gly), NCI-TCGA Cosmic COSV1004, cosmic curated COSV10041, Variant assessed as somatic; moderate impact.
- G86V (p.Gly86Val), gnomAD rs1446205816, REVEL 0.76, CADD 23.30
- G87D (p.Gly87Asp), rs145302631, ClinGen CA595703, ClinVar RCV002426327, ClinVar RCV003102025, REVEL 0.78, CADD 24.20, Uncertain significance, Inborn genetic diseases; Homocystinuria due to methylene tetrahydrofolate reduct
- G87V (p.Gly87Val), rs145302631, ClinGen CA338422687, ClinVar RCV002808858, 1000Genomes rs145302631, REVEL 0.89, CADD 26.40, Uncertain significance, Inborn genetic diseases
- P88L (p.Pro88Leu), cosmic curated COSV56743, REVEL 0.96, CADD 28.30
- L89H (p.Leu89His), Ensembl rs373609128
- L89V (p.Leu89Val), rs1042792109, ClinGen CA17974723, ClinVar RCV001308833, TOPMed rs1042792109, Uncertain significance, Homocystinuria due to methylene tetrahydrofolate reductase deficiency
- I91T (p.Ile91Thr), Ensembl rs1644381008, REVEL 0.72, CADD 24.00
- I91V (p.Ile91Val), rs761545364, ClinGen CA595701, ClinVar RCV002431252, ClinVar RCV003102140, REVEL 0.19, CADD 11.20, Uncertain significance, Inborn genetic diseases; Homocystinuria due to methylene tetrahydrofolate reduct
- V93A (p.Val93Ala), Ensembl rs1644380829, REVEL 0.93, CADD 26.70
- V93G (p.Val93Gly), cosmic curated COSV56743
- V93L (p.Val93Leu), cosmic curated COSV56743
- V93M (p.Val93Met), rs1239688333, ClinGen CA338422634, ClinVar RCV003126347, TOPMed rs1239688333, REVEL 0.76, CADD 23.90, Uncertain significance, not provided
- W95* (p.Trp95Ter), Ensembl rs2100568509
- W95C (p.Trp95Cys), TOPMed rs1338194007
- H96P (p.His96Pro), Ensembl rs1570492723
- H96Y (p.His96Tyr), gnomAD rs1345151194, REVEL 0.89, CADD 24.60
- A98V (p.Ala98Val), Ensembl rs1557769229
- D100E (p.Asp100Glu), Ensembl rs1570492659, REVEL 0.36, CADD 18.70
- D100N (p.Asp100Asn), gnomAD rs1296179669, REVEL 0.32, CADD 22.80
- P101L (p.Pro101Leu), cosmic curated COSV10461
- P101R (p.Pro101Arg), NCI-TCGA TCGA novel, Variant assessed as somatic; moderate impact.
- S103* (p.Ser103Ter), Ensembl rs1644380045
- S103L (p.Ser103Leu), Ensembl rs1644380045, REVEL 0.73, CADD 29.00
- S103P (p.Ser103Pro), TOPMed rs1644380115, REVEL 0.72, CADD 28.30
- D104E (p.Asp104Glu), Ensembl rs1557769192
- D104V (p.Asp104Val), Ensembl rs1644379939
- D104Y (p.Asp104Tyr), NCI-TCGA Cosmic COSV5673, cosmic curated COSV56739, REVEL 0.73, CADD 26.30, Variant assessed as somatic; moderate impact.
- K105R (p.Lys105Arg), ExAC rs779696019, TOPMed rs779696019, gnomAD rs779696019, REVEL 0.68, CADD 25.50
- E106* (p.Glu106Ter), cosmic curated COSV56742
- E106D (p.Glu106Asp), NCI-TCGA TCGA novel, Variant assessed as somatic; moderate impact.
- E106V (p.Glu106Val), Ensembl rs756875135, REVEL 0.57, CADD 24.20
- T107P (p.Thr107Pro), Ensembl rs1570492613
- T107S (p.Thr107Ser), NCI-TCGA TCGA novel, Variant assessed as somatic; moderate impact.
- S108T (p.Ser108Thr), gnomAD rs1304056030, REVEL 0.50, CADD 22.10
- S108Y (p.Ser108Tyr), cosmic curated COSV10041
- S109F (p.Ser109Phe), rs745779146, ExAC rs745779146, gnomAD rs745779146, REVEL 0.94, CADD 29.50, Variant assessed as somatic; moderate impact.
- M110I (p.Met110Ile), rs781158269, ClinGen CA595693, ClinVar RCV000693573, ClinVar RCV003411613, REVEL 0.55, CADD 22.40, Likely benign, Homocystinuria due to methylene tetrahydrofolate reductase deficiency
- M110L (p.Met110Leu), TOPMed rs1314255798, REVEL 0.39, CADD 19.50
- M110T (p.Met110Thr), TOPMed rs1644379394
- M110V (p.Met110Val), TOPMed rs1314255798, REVEL 0.46, CADD 21.80
- M111I (p.Met111Ile), NCI-TCGA Cosmic COSV5674, cosmic curated COSV56740, 1000Genomes rs577135269, ExAC rs577135269, REVEL 0.25, CADD 18.30, Variant assessed as somatic; moderate impact.
- M111T (p.Met111Thr), ExAC rs756962297, gnomAD rs756962297, REVEL 0.27, CADD 18.30
- I112V (p.Ile112Val), rs1421914910, ClinGen CA338422506, ClinVar RCV001923959, gnomAD rs1421914910, REVEL 0.58, CADD 22.70, Uncertain significance, Homocystinuria due to methylene tetrahydrofolate reductase deficiency
- A113P (p.Ala113Pro), ESP rs147257424, ExAC rs147257424, TOPMed rs147257424, gnomAD rs147257424, REVEL 0.95, CADD 27.20, Pathogenic, in MTHFRD
- A113S (p.Ala113Ser), rs147257424, ClinGen CA595689, ClinVar RCV001838859, ESP rs147257424, REVEL 0.78, CADD 23.80, Likely pathogenic, Homocystinuria due to methylene tetrahydrofolate reductase deficiency
- A113T (p.Ala113Thr), rs147257424, ClinGen CA198574, ClinVar RCV000167592, ClinVar RCV003462247, REVEL 0.90, CADD 26.70, Pathogenic/Likely pathogenic, Neural tube defects, folate-sensitive; Homocystinuria due to methylene tetrahydr
- A113V (p.Ala113Val), NCI-TCGA TCGA novel, Variant assessed as somatic; moderate impact., in MTHFRD
- S114G (p.Ser114Gly), rs1199277582, ClinGen CA338422496, ClinVar RCV004523875, TOPMed rs1199277582, REVEL 0.35, CADD 22.60, Uncertain significance, Inborn genetic diseases
- T115P (p.Thr115Pro), Ensembl rs1570492449
- T115S (p.Thr115Ser), Ensembl rs1644378826, REVEL 0.50, CADD 22.30
- A116T (p.Ala116Thr), rs1056919085, ClinGen CA17974700, ClinVar RCV001808302, ClinVar RCV003464148, REVEL 0.79, CADD 24.00, Conflicting interpretations, not provided; Homocystinuria due to methylene tetrahydrofolate reductase deficie
- V117A (p.Val117Ala), TOPMed rs1163498568
- V117M (p.Val117Met), gnomAD rs1200746244, REVEL 0.48, CADD 16.90
- N118T (p.Asn118Thr), Ensembl rs1570492369
- Y119F (p.Tyr119Phe), TOPMed rs1276034428, gnomAD rs1276034428, REVEL 0.32, CADD 22.70
- Y119S (p.Tyr119Ser), TOPMed rs1276034428, gnomAD rs1276034428
- C120W (p.Cys120Trp), 1000Genomes rs576446829, TOPMed rs576446829, REVEL 0.74, CADD 23.70
- C120Y (p.Cys120Tyr), ExAC rs774066396, gnomAD rs774066396, REVEL 0.72, CADD 23.00
- E123D (p.Glu123Asp), gnomAD rs1314724398, REVEL 0.51, CADD 13.90, Uncertain significance, Inborn genetic diseases
- T124I (p.Thr124Ile), ExAC rs768248826, gnomAD rs768248826, REVEL 0.78, CADD 26.30
- T124P (p.Thr124Pro), Ensembl rs1570492288
- T124S (p.Thr124Ser), ExAC rs768248826, gnomAD rs768248826, REVEL 0.54, CADD 22.90
- I125V (p.Ile125Val), ExAC rs762688654, gnomAD rs762688654, REVEL 0.30, CADD 7.17
- H127Y (p.His127Tyr), rs769381688, ClinGen CA198577, NCI-TCGA Cosmic COSV1004, cosmic curated COSV10041, REVEL 0.97, CADD 27.30, Conflicting interpretations, not specified; Homocystinuria due to methylene tetrahydrofolate reductase defici
- M128V (p.Met128Val), rs202095816, ClinGen CA595678, ClinVar RCV001067667, 1000Genomes rs202095816, REVEL 0.46, CADD 22.30, Uncertain significance, Homocystinuria due to methylene tetrahydrofolate reductase deficiency
Public MTHFR analysis runs
- MTHFR analysis run — MTHFR (1,345 variants) — completed 2026-08-21