TUBB3 (Tubulin beta-3 chain) variants and mutations
TUBB3 (also known as Tubulin beta-3 chain) is a human protein-coding gene encoding a tubulin beta-3 chain protein. It forms neuronal microtubules required for axon growth, guidance, and intracellular transport. Heterozygous pathogenic variants can cause congenital fibrosis of the extraocular muscles type 3 and broader tubulinopathy phenotypes with brain and cranial-nerve abnormalities. This analysis covers 459 TUBB3 variants and mutations. Of these, 73% have computational variant effect predictions. Disease context includes fibrosis of extraocular muscles, congenital, 3A, with or without extraocular inv, Cortical dysgenesis with pontocerebellar hypoplasia due to TUBB3 mutation, and complex cortical dysplasia with other brain malformations 1. Example TUBB3 variants include R2K, R2M, and R2W.
Variant analysis overview
- Gene: TUBB3
- Protein: Tubulin beta-3 chain
- UniProt accession: Q13509
- Organism: Homo sapiens
- Variants analyzed: 459
- Variant scope: all variants
- Completed: 2026-08-22
Variant and mutation evidence
- Variant composition: 348 unspecified-consequence records; 63 missense variants; 9 frameshift variants; 17 synonymous variants; 6 stop-gained variants; 1 splice-region variants; 15 substitution
- Prediction scores: 335 variants have prediction scores (73% of the analyzed set).
Clinical, disease, and population context
- Disease context: 25 disease associations are represented. Top associations: fibrosis of extraocular muscles, congenital, 3A, with or without extraocular inv, Cortical dysgenesis with pontocerebellar hypoplasia due to TUBB3 mutation, complex cortical dysplasia with other brain malformations 1, congenital fibrosis of the extraocular muscles, breast cancer, neoplasm, prostate cancer, non-small cell lung carcinoma, breast carcinoma, Hodgkins lymphoma, TUBB3-related tubulinopathy, breast neoplasm.
Protein structure and variant hotspots
- Protein features: 22 binding sites; 3 post-translational modification sites.
- PTM context: 6 variants overlap post-translational modification sites.
Data sources
Evidence in this analysis draws on EBI Proteins Variation, UniProt, gnomAD v4, EuropePMC, 3D Hotspot Analysis, Interaction Network Analysis, Protein Data Bank, AlphaFold DB, gnomAD constraint, Open Targets, ClinGen, MaveDB, LitVar.
Notable TUBB3 variants
Examples include R2K, R2M, R2W, R2G, R2R, R2T, R2S, E3D. Listed records include available protein-change notation, database identifiers, clinical classifications, computational predictions, population evidence, experimental measurements, and disease context.
- R2K (p.Arg2Lys), rs2544615173, ClinGen CA397466776, ClinVar RCV003038132, ClinVar RCV003322637, REVEL 0.33, CADD 25.00, Conflicting interpretations, not provided; Complex cortical dysplasia with other brain malformations 1
- R2M (p.Arg2Met), rs2544615173, ClinGen CA397466785, ClinVar RCV003578817, REVEL 0.48, CADD 31.00, Uncertain significance, not provided
- R2W (p.Arg2Trp), gnomAD 16-89923405-A-T, REVEL 0.73, CADD 28.90
- R2G (p.Arg2Gly), gnomAD 16-89923405-A-G, REVEL 0.64, CADD 27.10
- R2R (p.Arg2Arg), gnomAD 16-89923405-A-C, CADD 16.70
- R2T (p.Arg2Thr), gnomAD 16-89923406-G-C, REVEL 0.52, CADD 29.40
- R2S (p.Arg2Ser), gnomAD 16-89923407-G-T, REVEL 0.52, CADD 28.60
- E3D (p.Glu3Asp), rs2144399229, ClinGen CA397466801, cosmic curated COSV10513, ClinVar RCV001752651, REVEL 0.67, CADD 28.10, Uncertain significance, not provided
- E3K (p.Glu3Lys), ExAC rs754904954, gnomAD rs754904954, REVEL 0.75, CADD 32.00
- E3R (p.Glu3Arg), gnomAD 16-89923405-AG-A, CADD 32.00
- E3* (p.Glu3Ter), gnomAD 16-89923408-G-T, CADD 44.00
- E3A (p.Glu3Ala), gnomAD 16-89923409-A-C, REVEL 0.77, CADD 32.00
- E3G (p.Glu3Gly), gnomAD 16-89923409-A-G, REVEL 0.78, CADD 33.00
- E3E (p.Glu3Glu), gnomAD 16-89923410-G-A, CADD 15.80
- I4F (p.Ile4Phe), 1000Genomes rs2144399233, REVEL 0.81, CADD 32.00
- I4V (p.Ile4Val), gnomAD 16-89923411-A-G, REVEL 0.46, CADD 28.10
- I4T (p.Ile4Thr), gnomAD 16-89923412-T-C, REVEL 0.73, CADD 25.90
- I4N (p.Ile4Asn), gnomAD 16-89923412-T-A, REVEL 0.83, CADD 32.00
- I4I (p.Ile4Ile), gnomAD 16-89923413-C-A, CADD 16.30
- V5L (p.Val5Leu), ExAC rs781135980, TOPMed rs781135980, gnomAD rs781135980, REVEL 0.33, CADD 24.00
- V5A (p.Val5Ala), gnomAD 16-89923413-CGT-C, CADD 32.00
- V5M (p.Val5Met), gnomAD 16-89923414-G-A, REVEL 0.57, CADD 26.50
- V5E (p.Val5Glu), gnomAD 16-89923415-T-A, REVEL 0.77, CADD 31.00
- V5V (p.Val5Val), gnomAD 16-89923416-G-T, CADD 14.80
- H6Y (p.His6Tyr), gnomAD 16-89923417-C-T, REVEL 0.43, CADD 24.60
- H6N (p.His6Asn), gnomAD 16-89923417-C-A, REVEL 0.21, CADD 24.40
- H6L (p.His6Leu), gnomAD 16-89923418-A-T, REVEL 0.32, CADD 23.20
- H6R (p.His6Arg), gnomAD 16-89923418-A-G, REVEL 0.53, CADD 26.10
- H6Q (p.His6Gln), gnomAD 16-89923419-C-A, REVEL 0.32, CADD 23.30
- H6H (p.His6His), rs2029957353, gnomAD 16-89923419-C-T, CADD 15.50
- I7V (p.Ile7Val), cosmic curated COSV10732, TOPMed rs1237423262, gnomAD rs1237423262, REVEL 0.03, CADD 22.40
- I7F (p.Ile7Phe), gnomAD 16-89923420-A-T, REVEL 0.46, CADD 24.50
- I7N (p.Ile7Asn), gnomAD 16-89923421-T-A, REVEL 0.55, CADD 32.00
- I7T (p.Ile7Thr), gnomAD 16-89923421-T-C, REVEL 0.57, CADD 26.40
- I7I (p.Ile7Ile), rs1360904972, gnomAD 16-89923422-C-A, CADD 16.20
- Q8K (p.Gln8Lys), Ensembl rs866533192, REVEL 0.57, CADD 27.40
- Q8R (p.Gln8Arg), gnomAD 16-89923421-TC-T, CADD 25.40
- Q8* (p.Gln8Ter), gnomAD 16-89923423-C-T, CADD 39.00
- Q8E (p.Gln8Glu), gnomAD 16-89923423-C-G, REVEL 0.48, CADD 24.10
- Q8P (p.Gln8Pro), gnomAD 16-89923424-A-C, REVEL 0.66, CADD 25.50
- Q8L (p.Gln8Leu), gnomAD 16-89923424-A-T, REVEL 0.73, CADD 31.00
- Q8H (p.Gln8His), gnomAD 16-89923425-G-T, REVEL 0.43, CADD 24.80
- Q8Q (p.Gln8Gln), gnomAD 16-89923425-G-A, CADD 15.60
- A9D (p.Ala9Asp), TOPMed rs2029957696, REVEL 0.64, CADD 28.50, Uncertain significance, not specified
- A9S (p.Ala9Ser), gnomAD 16-89923426-G-T, REVEL 0.36, CADD 27.50
- A9T (p.Ala9Thr), gnomAD 16-89923426-G-A, REVEL 0.32, CADD 23.30
- A9V (p.Ala9Val), gnomAD 16-89923427-C-T, REVEL 0.33, CADD 23.20
- A9A (p.Ala9Ala), rs376463892, gnomAD 16-89923428-C-T, CADD 16.90
- G10A (p.Gly10Ala), gnomAD 16-89923428-CG-C, CADD 32.00
- G10C (p.Gly10Cys), gnomAD 16-89923429-G-T, REVEL 0.95, CADD 33.00
- G10S (p.Gly10Ser), gnomAD 16-89923429-G-A, REVEL 0.98, CADD 32.00
- G10D (p.Gly10Asp), gnomAD 16-89923430-G-A, REVEL 0.97, CADD 32.00
- G10V (p.Gly10Val), gnomAD 16-89923430-G-T, REVEL 0.96, CADD 32.00
- G10G (p.Gly10Gly), rs1178928369, gnomAD 16-89923431-C-G, CADD 17.20
- Q11H (p.Gln11His), rs2144399284, ClinGen CA397467036, ClinVar RCV001723250, Ensembl rs2144399284, REVEL 0.63, CADD 26.10, Uncertain significance, X-linked hydrocephalus syndrome
- Q11K (p.Gln11Lys), rs2544615220, ClinGen CA397467006, ClinVar RCV003716704, REVEL 0.69, CADD 24.80, Uncertain significance, not provided
- Q11S (p.Gln11Ser), gnomAD 16-89923430-GC-G, CADD 28.00
- Q11* (p.Gln11Ter), gnomAD 16-89923432-C-T, CADD 39.00
- Q11R (p.Gln11Arg), gnomAD 16-89923433-A-G, REVEL 0.53, CADD 25.10
- Q11L (p.Gln11Leu), gnomAD 16-89923433-A-T, REVEL 0.65, CADD 25.70
- Q11Q (p.Gln11Gln), gnomAD 16-89923434-G-A, CADD 14.90
- C12S (p.Cys12Ser), gnomAD 16-89923435-T-A, REVEL 0.51, CADD 25.50
- C12R (p.Cys12Arg), gnomAD 16-89923435-T-C, REVEL 0.68, CADD 26.80
- C12W (p.Cys12Trp), gnomAD 16-89923435-TGC-T, CADD 32.00
- C12Y (p.Cys12Tyr), gnomAD 16-89923436-G-A, REVEL 0.87, CADD 32.00
- C12F (p.Cys12Phe), gnomAD 16-89923436-G-T, REVEL 0.82, CADD 26.20
- C12C (p.Cys12Cys), rs2029957972, gnomAD 16-89923437-C-T, CADD 16.50
- C12* (p.Cys12Ter), gnomAD 16-89923437-C-A, CADD 36.00
- G13C (p.Gly13Cys), gnomAD 16-89923438-G-T, REVEL 0.86, CADD 32.00
- G13S (p.Gly13Ser), gnomAD 16-89923438-G-A, REVEL 0.85, CADD 32.00
- G13A (p.Gly13Ala), gnomAD 16-89923439-G-C, REVEL 0.91, CADD 31.00
- G13V (p.Gly13Val), gnomAD 16-89923439-G-T, REVEL 0.97, CADD 32.00
- G13D (p.Gly13Asp), gnomAD 16-89923439-G-A, REVEL 0.92, CADD 32.00
- G13G (p.Gly13Gly), gnomAD 16-89923440-C-G, CADD 16.40
- N14Y (p.Asn14Tyr), NCI-TCGA TCGA novel, Variant assessed as somatic; moderate impact.
- N14D (p.Asn14Asp), gnomAD 16-89923441-A-G, REVEL 0.68, CADD 32.00
- N14I (p.Asn14Ile), gnomAD 16-89923442-A-T, REVEL 0.75, CADD 32.00
- N14S (p.Asn14Ser), gnomAD 16-89923442-A-G, REVEL 0.53, CADD 25.00
- N14K (p.Asn14Lys), gnomAD 16-89923443-C-A, REVEL 0.75, CADD 29.90
- N14N (p.Asn14Asn), rs769965307, gnomAD 16-89923443-C-T, CADD 16.50
- Q15R (p.Gln15Arg), gnomAD 16-89923442-AC-A, CADD 32.00
- Q15* (p.Gln15Ter), gnomAD 16-89923444-C-T, CADD 42.00
- Q15E (p.Gln15Glu), gnomAD 16-89923444-C-G, REVEL 0.54, CADD 24.80
- Q15K (p.Gln15Lys), gnomAD 16-89923444-C-A, REVEL 0.55, CADD 24.90
- Q15L (p.Gln15Leu), gnomAD 16-89923445-A-T, REVEL 0.61, CADD 32.00
- Q15Q (p.Gln15Gln), gnomAD 16-89923446-G-A, CADD 15.50
- Q15H (p.Gln15His), gnomAD 16-89923446-G-T, REVEL 0.66, CADD 24.70
- I16F (p.Ile16Phe), rs2144399297, ClinGen CA397467142, ClinVar RCV001575554, Ensembl rs2144399297, REVEL 0.79, CADD 32.00, Uncertain significance, not provided
- I16M (p.Ile16Met), TOPMed rs1432450375, gnomAD rs1432450375
- I16V (p.Ile16Val), gnomAD 16-89923447-A-G, REVEL 0.20, CADD 24.00
- I16N (p.Ile16Asn), gnomAD 16-89923448-T-A, REVEL 0.81, CADD 32.00
- I16T (p.Ile16Thr), gnomAD 16-89923448-T-C, REVEL 0.75, CADD 32.00
- I16I (p.Ile16Ile), rs1432450375, gnomAD 16-89923449-C-T, CADD 15.90
- G17R (p.Gly17Arg), rs778032071, ClinGen CA8255884, ClinVar RCV003689760, ExAC rs778032071, REVEL 0.78, CADD 33.00, Likely benign, not provided
- G17V (p.Gly17Val), rs2544615257, ClinGen CA397467184, ClinVar RCV003159445, REVEL 0.74, CADD 30.00, Uncertain significance, not provided
- G17W (p.Gly17Trp), gnomAD 16-89923450-G-T, REVEL 0.82, CADD 33.00
- G17E (p.Gly17Glu), gnomAD 16-89923451-G-A, REVEL 0.80, CADD 29.90
- G17G (p.Gly17Gly), gnomAD 16-89923452-G-T, CADD 15.60
- A18P (p.Ala18Pro), gnomAD 16-89923449-CG-C, CADD 33.00
- A18T (p.Ala18Thr), gnomAD 16-89923453-G-A, REVEL 0.11, CADD 23.40
- A18S (p.Ala18Ser), gnomAD 16-89923453-G-T, REVEL 0.09, CADD 22.90
- A18V (p.Ala18Val), gnomAD 16-89923454-C-T, REVEL 0.39, CADD 25.60
- A18D (p.Ala18Asp), gnomAD 16-89923454-C-A, REVEL 0.45, CADD 29.40
- A18A (p.Ala18Ala), gnomAD 16-89923455-C-T, CADD 18.60
- K19S (p.Lys19Ser), gnomAD 16-89923453-GC-G, CADD 32.00
- K19* (p.Lys19Ter), gnomAD 16-89923456-A-T, CADD 47.00
- K19E (p.Lys19Glu), gnomAD 16-89923456-A-G, REVEL 0.35, CADD 27.60
- K19R (p.Lys19Arg), gnomAD 16-89923457-A-G, REVEL 0.38, CADD 29.20
- K19M (p.Lys19Met), gnomAD 16-89923457-A-T, REVEL 0.67, CADD 34.00
- K19T (p.Lys19Thr), gnomAD 16-89923457-A-C, REVEL 0.63, CADD 33.00
- K19K (p.Lys19Lys), rs2029958305, gnomAD 16-89923458-G-A, CADD 26.20
- K19N (p.Lys19Asn), gnomAD 16-89923458-G-T, REVEL 0.56, CADD 36.00
- I24T (p.Ile24Thr), rs1567763964, ClinGen CA397470692, ClinVar RCV000729110, Ensembl rs1567763964, REVEL 0.75, CADD 29.20, Uncertain significance, not provided
- D31A (p.Asp31Ala), Ensembl rs1597423178
- D31N (p.Asp31Asn), cosmic curated COSV59248, gnomAD rs2030317038, REVEL 0.21, CADD 27.50
- P32A (p.Pro32Ala), gnomAD rs2030317179, REVEL 0.26, CADD 24.20
- P32R (p.Pro32Arg), rs767364590, ClinGen CA8255940, ClinVar RCV003988562, ExAC rs767364590, REVEL 0.52, CADD 25.20, Uncertain significance, not specified
- P32T (p.Pro32Thr), gnomAD rs2030317179, REVEL 0.36, CADD 24.50
- N35T (p.Asn35Thr), gnomAD rs1317414525, REVEL 0.20, CADD 18.60
- Y36C (p.Tyr36Cys), rs2151091993, ClinGen CA397470920, ClinVar RCV003006335, AlphaMissense 0.37, MetaLR 0.25, Uncertain significance, not provided
- Y36F (p.Tyr36Phe), Ensembl rs2151091993
- Y36N (p.Tyr36Asn), TOPMed rs2030317630
- V37M (p.Val37Met), rs372720472, ClinGen CA8255943, ClinVar RCV000432537, ESP rs372720472, REVEL 0.11, CADD 19.60, Conflicting interpretations, not provided
- D39N (p.Asp39Asn), rs761851735, ClinGen CA8255945, ClinVar RCV003428016, ExAC rs761851735, REVEL 0.15, CADD 26.20, Uncertain significance, not provided
- S40L (p.Ser40Leu), rs2030318148, ClinGen CA397470983, ClinVar RCV002855786, Ensembl rs2030318148, REVEL 0.35, CADD 23.10, Uncertain significance, Inborn genetic diseases
- Q43E (p.Gln43Glu), TOPMed rs1423741574, gnomAD rs1423741574, REVEL 0.47, CADD 23.80
- Q43R (p.Gln43Arg), TOPMed rs1338930472, gnomAD rs1338930472, REVEL 0.55, CADD 23.80
- R46Q (p.Arg46Gln), rs1333706172, ClinGen CA397471030, cosmic curated COSV10020, ClinVar RCV001843614, REVEL 0.62, CADD 27.10, Pathogenic, not provided
- R46W (p.Arg46Trp), rs1555625363, ClinGen CA397471028, ClinVar RCV000656083, ClinVar RCV001824143, AlphaMissense 0.95, MetaLR 0.71, Conflicting interpretations, Developmental disorder; not provided; Complex cortical dysplasia with other brai
- I47T (p.Ile47Thr), TOPMed rs2030318802
- S48G (p.Ser48Gly), TOPMed rs113165321, REVEL 0.27, CADD 23.10
- S48I (p.Ser48Ile), gnomAD rs1464698913, REVEL 0.43, CADD 23.10
- V49I (p.Val49Ile), gnomAD rs1398545228, REVEL 0.47, CADD 23.50
- N52K (p.Asn52Lys), rs1343459898, ClinGen CA397471104, ClinVar RCV003838427, Uncertain significance, not provided
- E53K (p.Glu53Lys), Ensembl rs2151092013, REVEL 0.56, CADD 26.10, Uncertain significance, not provided
- S55C (p.Ser55Cys), ExAC rs781758598, gnomAD rs781758598
- H57R (p.His57Arg), ExAC rs761094494, gnomAD rs761094494, REVEL 0.12, CADD 22.10
- V60L (p.Val60Leu), rs2151092282, ClinGen CA397471203, ClinVar RCV001775244, Ensembl rs2151092282, AlphaMissense 0.99, MetaLR 0.46, Likely pathogenic, Complex cortical dysplasia with other brain malformations 1
- P61S (p.Pro61Ser), ESP rs375486300, ExAC rs375486300, gnomAD rs375486300
- R62* (p.Arg62Ter), cosmic curated COSV59247, TOPMed rs1423200485, gnomAD rs1423200485, CADD 42.00
- R62G (p.Arg62Gly), TOPMed rs1423200485, gnomAD rs1423200485, REVEL 0.82, CADD 25.30, Uncertain significance, Complex cortical dysplasia with other brain malformations 1
- R62Q (p.Arg62Gln), rs864321714, ClinGen CA347966, NCI-TCGA Cosmic COSV5924, cosmic curated COSV59248, REVEL 0.89, CADD 32.00, Likely pathogenic, not provided
- I64V (p.Ile64Val), rs797046076, ClinGen CA205814, ClinVar RCV000192764, Ensembl rs797046076, AlphaMissense 0.10, MetaLR 0.07, Uncertain significance, not specified
- D67V (p.Asp67Val), rs2544625607, ClinGen CA397471243, ClinVar RCV003314781, Uncertain significance, not provided
- E69K (p.Glu69Lys), rs2544625618, ClinGen CA397471252, ClinVar RCV002284680, Uncertain significance, not provided
- G71A (p.Gly71Ala), rs2151092293, ClinGen CA397471269, ClinVar RCV001817699, Ensembl rs2151092293, AlphaMissense 0.80, MetaLR 0.49, Likely pathogenic, not provided
- G71R (p.Gly71Arg), rs864321715, ClinGen CA347960, NCI-TCGA Cosmic COSV5924, cosmic curated COSV59247, REVEL 0.63, CADD 29.70, Pathogenic/Likely pathogenic, TUBB3-related tubulinopathy; not provided; Complex cortical dysplasia with other
- M73T (p.Met73Thr), TOPMed rs1415414936, gnomAD rs1415414936
- D74G (p.Asp74Gly), rs2030344761, ClinGen CA397471290, ClinVar RCV001092319, Ensembl rs2030344761, AlphaMissense 0.98, MetaLR 0.55, Uncertain significance, not provided
- D74N (p.Asp74Asn), Ensembl rs1567764331
- R77C (p.Arg77Cys), rs2030345139, ClinGen CA397471310, NCI-TCGA Cosmic COSV1002, cosmic curated COSV10020, REVEL 0.59, CADD 27.50, Uncertain significance, Fibrosis of extraocular muscles, congenital, 3A, with or without extraocular inv
- R77H (p.Arg77His), cosmic curated COSV59247, gnomAD rs1216251571, REVEL 0.53, CADD 25.60
- S78L (p.Ser78Leu), ExAC rs777573566, gnomAD rs777573566, REVEL 0.42, CADD 26.80, Likely pathogenic, Fibrosis of extraocular muscles, congenital, 3A, with or without extraocular inv
- A80S (p.Ala80Ser), TOPMed rs2030345543
- F81S (p.Phe81Ser), TOPMed rs1383940374, gnomAD rs1383940374, REVEL 0.42, CADD 27.20
- G82A (p.Gly82Ala), cosmic curated COSV10881, Ensembl rs2151092305
- L84I (p.Leu84Ile), Ensembl rs2030345780, REVEL 0.14, CADD 14.60
- R86G (p.Arg86Gly), gnomAD rs1294173530, REVEL 0.54, CADD 25.10
- I91V (p.Ile91Val), rs2544625679, ClinGen CA397471404, ClinVar RCV002291444, Uncertain significance, not provided
- Q94H (p.Gln94His), TOPMed rs1187620930
- G98C (p.Gly98Cys), Ensembl rs587784505, Pathogenic
- G98S (p.Gly98Ser), rs587784505, ClinGen CA213295, NCI-TCGA Cosmic COSV1002, cosmic curated COSV10020, AlphaMissense 0.91, MetaLR 0.68, Pathogenic/Likely pathogenic, not provided; Complex cortical dysplasia with other brain malformations 1; Intel
- H105N (p.His105Asn), rs2544627057, ClinGen CA397474185, ClinVar RCV002291127, Likely pathogenic, Complex cortical dysplasia with other brain malformations 1
- Y106* (p.Tyr106Ter), rs1220586790, ClinGen CA397474199, ClinVar RCV001760660, TOPMed rs1220586790, Uncertain significance
- T107M (p.Thr107Met), rs2030395720, ClinGen CA397474204, cosmic curated COSV59247, ClinVar RCV002261560, REVEL 0.70, CADD 25.50, Uncertain significance, not provided
- G109R (p.Gly109Arg), rs2544627074, ClinGen CA397474215, ClinVar RCV004548964, Uncertain significance, TUBB3-related disorder
- A110T (p.Ala110Thr), rs2544627086, ClinGen CA397474220, ClinVar RCV003129335, Uncertain significance, not provided
- A110V (p.Ala110Val), cosmic curated COSV59248, TOPMed rs1186388176, gnomAD rs1186388176, REVEL 0.58, CADD 25.30
- S115L (p.Ser115Leu), rs2544627103, ClinGen CA397474258, ClinVar RCV003482197, REVEL 0.44, CADD 25.60, Uncertain significance, Complex cortical dysplasia with other brain malformations 1
- L117M (p.Leu117Met), cosmic curated COSV59247, ExAC rs754239056, gnomAD rs754239056, REVEL 0.13, CADD 22.80
- L117P (p.Leu117Pro), rs2544627119, ClinGen CA397474267, ClinVar RCV003333595, Uncertain significance, Complex cortical dysplasia with other brain malformations 1
- V120A (p.Val120Ala), gnomAD rs1162346170, REVEL 0.70, CADD 28.50
- R121Q (p.Arg121Gln), rs1229540455, NCI-TCGA Cosmic COSV5924, cosmic curated COSV59249, TOPMed rs1229540455, REVEL 0.82, CADD 32.00, Uncertain significance, not provided
- C124G (p.Cys124Gly), Ensembl rs1597424667, REVEL 0.27, CADD 24.50
- C124S (p.Cys124Ser), rs2544627184, ClinGen CA397474314, ClinVar RCV003983793, REVEL 0.17, CADD 22.60, Uncertain significance, TUBB3-related tubulinopathy
- N126K (p.Asn126Lys), gnomAD rs1597424672, REVEL 0.12, CADD 17.50
- N126T (p.Asn126Thr), rs2544627187, ClinGen CA397474329, ClinVar RCV003129251, Uncertain significance, not provided
- C127* (p.Cys127Ter), ESP rs145375049, ExAC rs145375049, TOPMed rs145375049, gnomAD rs145375049, CADD 37.00, Likely benign
- D128N (p.Asp128Asn), TOPMed rs998987819, REVEL 0.78, CADD 31.00
- C129Y (p.Cys129Tyr), rs2151092738, ClinGen CA397474352, ClinVar RCV001765377, Ensembl rs2151092738, AlphaMissense 0.90, MetaLR 0.53, Likely pathogenic, not provided
Public TUBB3 analysis runs
- TUBB3 analysis run — TUBB3 (459 variants) — completed 2026-08-22